In order to bring you the best possible user experience, this site uses Javascript. If you are seeing this message, it is likely that the Javascript option in your browser is disabled. For optimal viewing of this site, please ensure that Javascript is enabled for your browser.
Did you know that your browser is out of date? To get the best experience using our website we recommend that you upgrade to a newer version. Learn more.

We use cookies to optimise the design of this website and make continuous improvement. By continuing your visit, you consent to the use of cookies. Learn more

Amsterdam Cardiovascular Development Meeting: Abstract on Hes1...

Dissecting progenitor cell contributions to the developing heart: a potential role of Hes1

Mayyasa Rammah, Magali Théveniau-Ruissy, Francesca Rochais* and Robert G Kelly*

Aix-Marseille University, Developmental Biology Institute of Marseilles, CNRS UMR7288, 13288, Marseilles, France.
*equal contribution

Cardiac progenitor cells of the second heart field (SHF) contribute to the poles of the elongating embryonic heart. Perturbation of SHF development leads to a spectrum of congenital heart defects. Recent evidence suggests that distinct regions of the heart are pre-patterned in the SHF. In particular, the myocardium at the base of the aorta and pulmonary trunk were shown to be prefigured in the outflow tract. For example the dell22q11.2 or DiGeorge syndrome gene Tbx1 is required in the SHF for development of the inferior wall of the embryonic outflow tract, giving rise to subpulmonary myocardium. By characterizing the expression of an enhancer trap transgene at the Hes1 locus, encoding a transcriptional repressor, we have identified a complementary Notch-dependent Hes1+ Tbx1- subpopulation of SHF cells giving rise to future subaortic myocardium. Using transcriptomic analysis on superior and inferior outflow tracts, we have characterized the distinct genetic signatures of future subaortic and subpulmonary myocardium and identified peroxisome proliferator activated receptor gamma (Pparg) among the genes enriched in future subpulmonary myocardium. We have also shown that Pparg acts as an upstream regulator in the cross-circuitry operating in superior and inferior OFT walls and plays a role in SHF cell addition to the OFT. Our genetic and explant analyses have revealed that Hes1, as a downstream target of Notch signaling pathway, controls the molecular signature of future subaortic myocardium through direct transcriptional repression of Pparg. Altogether, our study reveals distinct genetic regulatory networks controlling different progenitor cell contributions to the developing heart and identifies a crucial role of Hes1 in the regulation of cardiac progenitor cells fate.

The content of this article reflects the personal opinion of the author/s and is not necessarily the official position of the European Society of Cardiology.

Contact us

Working Group on Development, Anatomy & Pathology

European Society of Cardiology

European Heart House
Les Templiers
2035 Route des Colles
CS 80179 Biot

06903Sophia Antipolis, FR